RNA

Part:BBa_K4162005:Design

Designed by: Weiwen Chen   Group: iGEM22_Fudan   (2022-09-29)


Hammerhead ribozyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence of hammerhead ribozyme should be inserted between two coding sequence. By introducing self-cleaving ribozyme sequence, we can create polycistron in prokaryotes such as Escherichia coli.


Source

Termed hammerhead, one of the ribozyme families.

References